Figure 6
From: Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit
Functional assay of FcNAC1 gene by transient transformation of tobacco leaves. The ratio of LUC/REN activities obtained after Dual Luciferase interaction assay of FcNAC1 with the promoter sequences of FvDFR (Dihydroflavonol reductase, 1560 bp) (XP_004308377), FcEXP2 (Expansin 2, GenBank KC527027, 847 bp) and FcPL (Pectate lyase, GenBank KC527025, 1038 bp) in transiently transfected N. benthamiana leaves are shown. GUS represents the plasmid 35SPro-GUS (negative control). FvMYB10 (GenBank EU155163.1) and FvBHLH (Basic helix loop helix XP_004308377; 1932 bp) were used as positive controls of the technique. Bars represent the bioluminescent measure of 4 replicates ± standard deviation. Different letters indicate statistical differences (p ≤ 0.05) compared to respective GUS control.
