Figure 2
The SEC-SAXS analysis of CarD. (a) Scattering data obtained for CarD at 277 μM concentration. Inset shows the in-line SEC-trace of the CarD. CarD elutes as a single peak with no apparent signs of aggregation. FoXS web server analysis reveals that the theoretically calculated scattering profile from domain-swapped dimeric crystal structure (blue) matches well (χ = 1.23) with the experimentally observed scattering profile (black) whereas the monomeric CarD (red) shows relatively poor fit (χ = 1.48). (b) The linear low-q region (0.04–0.18) of the scattering curves were used for the Guinier analysis. (c) Kratky analysis of the CarD suggests CarD is well folded and flexible in solution. (d) Normalized pair distance distribution [P(r)] function suggests Dmax of around 72 Å for CarD.
