Figure 3

Immunohistochemical staining and double-immunofluorescence labeled microscopic images of ECIs in RCCs. Immunohistochemical staining showed that the ECIs were immunopositive for P62 (A), NBR1 (B), LC3 (C), BECN1 (D), and ATG5 (E), and immunonegative for LCK (arrows in G). Ub1 immunopositivity was detected in a small number of ECIs (arrows in F). Double-immunofluorescence staining of ECIs demonstrated co-localization of P62 with NBR1, LC3, BECN1 or ATG5 in ECIs (H).