Figure 4
Localization of organelle markers in ECIs of RCCs. Immunohistochemical staining showed that ECIs were intensively positive for PEX14 and CAT1 (two markers of peroxisomes, arrows in A and B). Some of the ECIs were positive for GM130 (a marker of Golgi apparatus, arrows in C). None of the ECIs exhibited immunoreactivity of LAMP1 and LAMP2 (two markers of lysosome or autolysosome, arrowheads in D and E), TOM20 (a marker of mitochondria, arrowheads in F), CALR (a marker of the endoplasmic reticulum, arrowheads in G), RPS6 (a marker of ribosomes, arrowheads in H), or RAB5A (a marker of early endosomes, arrowheads in I). Double- immunofluorescence labelling of ECIs revealed that most of the NBR1-positive ECIs were positive for PEX14 and CAT1, and that a portion of them displayed moderate GM130 staining (J).
