Figure 3
From: Eosinophils support adipocyte maturation and promote glucose tolerance in obesity
ΔdblGATA mice showed defective adipocyte enlargement and a decreased expression of genes involved in adipocyte maturation on a high-fat diet (HFD). (a) Haematoxylin and eosin staining of the perigonadal fat of wild-type (WT) and ΔdblGATA mice fed a HFD or chow diet. Images are representative of two independent experiments. Original magnification × 20. The scale bar represents 50 μm. (b,c) Adipocyte size and distribution. (b) Mean adipocyte size and (c) percentile distribution of adipocyte size. Three sections were examined in each group and 2–3 fields were selected from one section. *p < 0.05, **p < 0.01, ***p < 0.001 (Mann-Whitney test). (d and e) mRNA expression levels of (d) adipogenic and (e) adipokine genes in the perigonadal fat of mice on a HFD. *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t-test for Pparg, Cav1, Cd36, Cidec, and Lep, Mann-Whitney test for Cav2, Lpl, Slc2a4, and Adipoq). Graphs show the mean ± standard error of the mean.
