Figure 5

ΔdblGATA mice showed increased inflammatory responses in the perigonadal adipose tissue on a high fat diet (HFD). (a) Flow cytometric analysis of SiglecF⁻F4/80⁺ macrophages, CD3⁺CD4⁺ T cells, CD3⁺CD8⁺ T cells, and CD3⁻B220⁺ B cells in the perigonadal fat of wild-type (WT) and ΔdblGATA mice on a HFD. Representative dot plots are shown. *p < 0.05, ***p < 0.001 (Student’s t-test for macrophage, Mann-Whitney test for CD4 T cells, CD8 T cells, and B cells). (b) mRNA expression of innate (Il1b, Il6, and Tnf) and adaptive (Ifng, Il4, Il5, and Il13) immune cytokines in the perigonadal fat of mice on a HFD. *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t-test for Il1b, Il6, Tnf, and Il4, Mann-Whitney test for Ifng, Il5, and Il13). Graphs show the mean ± standard error of the mean.