Figure 4
From: Discovery of peptide probes to modulate oxytocin-type receptors of insects
Atosiban competitively antagonises inotocin-mediated IP1 accumulation in HEK293 cells expressing the T. castaneum inotocin receptor. (A) Concentration-dependent rightward displacement of the concentration response curve by atosiban. Cells were stimulated with varying concentrations of inotocin (1 pM – 10 µM, filled black circles) in the absence or presence of 100 nM (open red squares), 1 µM (open blue triangles), 3 µM (open green diamonds), and 10 µM (open purple circles) atosiban. (B) Schild analysis of antagonist shifts. Data are presented as mean ± S.E.M. of three independent experiments conducted in triplicate. pA2 = 7.03 ± 0.086, Schild Slope = 1.00 ± 0.085.
