Figure 2 | Scientific Reports

Figure 2

From: Graphene oxide polarizes iNKT cells for production of TGFβ and attenuates inflammation in an iNKT cell-mediated sepsis model

Figure 2

TLR4 signaling negatively impacts the suppressive effects of GO on α-GalCer-mediated immune responses. (a,b) C3H/HeN and C3H/HeJ mice were i.p. injected with α-GalCer (2 μg) or PBS control and were concurrently i.v. injected with either GO (50 μg) or PBS control. Fourteen hrs later, splenocytes were prepared from each experimental group. (a) Intracellular IFNγ (upper panels) and TNFα (lower panels) production in iNKT and NK cells was assessed by flow cytometry. The mean values ± SD are shown (unpaired two-tailed Student’s t-test; *P < 0.05, **P < 0.01, ***P < 0.001; n = 4 per group in the experiment). Two-way ANOVA (genotype × treatment) showed a significant difference between these two factors (#P < 0.05). (b) Expression of CD86 (upper panels) and CD40 (lower panels) in DCs and macrophages were assessed by flow cytometry. The mean values ± SD are shown (unpaired two-tailed Student’s t-test; *P < 0.05, **P < 0.01, ***P < 0.001; n = 4 per group in the experiment). Two-way ANOVA (genotype × treatment) showed a significant difference between these two factors (#P < 0.05 and ###P < 0.001).

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