Table 1 Primers sets used in this study for mRNA analysis and promoter analysis after chromatin immunoprecipitation.
From: Adolescent alcohol exposure epigenetically regulates CREB signaling in the adult amygdala
Primer name | Sequence (5′ to 3′) |
|---|---|
Primers for mRNA analysis via RT-qPCR | |
Creb1 mRNA forward | AGAAGCAGCACGAAAGAGAG |
Creb1 mRNA reverse | CACTGCCACTCTGTTCTCTAAA |
Cbp mRNA forward | TAATGGAGGCTGCCCAGTGTGTAA |
Cbp mRNA reverse | CTGGCGGAGCTTGTGTTTGATGTT |
p300 mRNA forward | AAACACCAGCAACGAGAGTACCGA |
p300 mRNA reverse | TCCATGGTGGCGTACAGTTTCTGA |
Hprt1 mRNA forward | TCCTCAGACCGCTTTTCCCGC |
Hprt1 mRNA reverse | TCATCATCACTAATCACGACGCTGG |
Gadph mRNA forward | ACAAGATGGTGAAGGTCGGTGTGA |
Gadph mRNA reverse | AGCTTCCCATTCTCAGCCTTGACT |
Primers for Chromatin Immunoprecipitation | |
Creb1 proximal promoter (+593) forward | AGTGTCTTGTACTCTGCCGTG |
Creb1 proximal promoter (+593) reverse | ACATAGTGGGGCACAGAGGT |
Creb1 promoter CRE site (+375) forward | GGGATCTGAAGCCAGAATCTCA |
Creb1 promoter CRE site (+375) reverse | TGCACCAGTCAGGTTCAGAAA |
Cbp distal promoter (+3014) forward | AAAGCTAGCAAGGCGGTAAG |
Cbp distal promoter (+3014) reverse | CCCTTCCAAAGTGTACGGTAAG |
Cbp proximal promoter (+590) forward | TCTAGGTCCTGTGCAGCCAT |
Cbp proximal promoter (+590) reverse | CGGTAGAATTCCTCGTGCTGA |
p300 distal promoter (+1536) forward | AGTGTCCACCGACCGAAAAT |
p300 distal promoter (+1536) reverse | GTCATTCGGTGGCTCCCTTT |
p300 proximal promoter (+198) forward | TCAGTGTTGCTGTACCCTCC |
p300 proximal promoter (+198) reverse | TGCGGACTCAACAGAAATGGT |
