Table 2 Comparison of eDNA detection distances observed in caged fish experiments (Exp) or observational studies (Obs) and those predicted by simulation considering a value of the vertical transfer of FPOM from the water column to the riverbed (Vdep) computed from selected publications (median value ± interquartile range, see Supplementary Table 4) The maximal predicted detection distance is defined as the distance for which only one mtDNA copy of a hypothetical MOTU quantity released upstream is still present in the water column (see Method section for explanation).
Reference | Method | DNA analysis | Waterflow (m3.s−1) | Wetted width (m) | Observed distance of detection | Predicted distance of detection (±Interquartile Q1–Q3) |
|---|---|---|---|---|---|---|
Jane et al. (2015) | Exp | qPCR | 0.003 | 1.19 | >239.5 m | 128 m (54–283) |
0.031 | 3.35 | >239.5 m | 443 m (186–979) | |||
Wilcox et al. (2016) | Exp | qPCR | 0.006 | 1.19 | 41 to 222 m | 235 m (98–518) |
Exp | 0.010 | 3.35 | 72 to 1,459 m | 137 m (57–303) | ||
Obs. | 0.059 | 2.88 | 100 to 900 m | 965 m (404–2131) | ||
Civade et al. (2016) | Obs. | meta barcoding | 0.170 | 1.80 | 1.7 to 3.6 km | 4.5 km (1.9–9.9) |
Deiner et Altermatt (2014) | Obs | qPCR | 3.520 | 14.00 | >9.1 km | 11.9 km (5–26.3) |
3.520 | 14.00 | >9.1 km | 12.8 km (5.4–28.3) | |||
3.790 | 14.00 | 1.6 to 9.1 km | 11.9 km (5–26.3) | |||
This study | Obs | meta barcoding | 436.00 | 132.00 | 130.0 km (120.7–141.1) | 156.1 km (65.4–344.9) |
