Figure 2 | Scientific Reports

Figure 2

From: Post-treatment with PT302, a long-acting Exendin-4 sustained release formulation, reduces dopaminergic neurodegeneration in a 6-Hydroxydopamine rat model of Parkinson’s disease

Figure 2

Pretreatment with PT302 reduces meth-mediated rotational behavior in hemiparkinsonian rats. Animals were treated with vehicle (n = 9), low dose PT302 (equivalent to Exendin-4 0.4 mg/kg every 2 weeks, n = 9) or high dose PT302 (equivalent to Exendin-4 2.0 mg/kg/ every 2 weeks, n = 10) starting 16 days prior to 6-OHDA lesioning. (a) Time line of study demonstrating PT302/vehicle dosing (day −16, −2, 12, 26 and 40 relative to day 0 when lesioning was performed), meth-mediated rotation was examined on days 20, 30 and 45 post-lesioning, a blood sample was taken on day 47 and animals were later euthanized. (b) Treatment with PT302 significantly reduced rotation (p = 0.018, F2, 87 = 4.309, [p < 0.001 in Fig. 2b] two way ANOVA). A post-hoc Newman-Keuls test indicated that the high dose of PT302 significantly attenuated meth-mediated rotation (p = 0.037). A non-significant decline was found between the vehicle and low dose PT302 groups (p = 0.156). (c) Exendin-4 plasma levels (pg/ml) were evaluated on day 47 for each animal, and were additionally evaluated for the presence of an anti-Exendin-4 antibody titer. Animals No. 1, 10 and 12 demonstrated a positive anti-Exendin-4 antibody titer (red circle).

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