Figure 4
From: Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors
iPSC-derived MKs differentiated in stirred flasks show increased DNA content and the capability to form proPLTs. Polyploidy of the harvested cells was analyzed quantitatively by flow cytometry after propidium iodide staining. Exemplary histograms show MK DNA contents (red) and iPSC DNA content (grey) (a). DAPI staining revealed polyploidy in fluorescence microscopic analyses (b). Formation of proPLTs was shown in cells from both differentiation setups (c). Quantification of proPLT forming cells (d) and proPLTs per proPLT forming cell (e) were performed. Graph is depicted as mean ± SD of n = 4.
