Figure 5
From: Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors
Irradiation does not affect proPLT and PLT formation from iPSC-derived megakaryocytes produced in cell-MC differentiation. MKs produced from iPSCs in stirred cell-MC cultivation, both non-treated (upper row) and irradiated (lower row) were seeded for 24 h on poly-l-lysine-coated coverslips. Representative immunofluorescence images of cells labelled for β-tubulin (green) and CD61 (magenta) show comparable proPLT structures between the two conditions (a). At least ten images were analyzed and the frequency of proPLT forming cells (b) or the number of proPLTs per proPLT forming cell (c) was not affected by irradiation. One day after irradiation, PLTs produced in vitro from cell-MC MKs were identified by size and CD41 expression by flow cytometry and further analyzed for the co-expression of CD42a and CD61. No difference between non-treated and irradiated cells was detectable (d). PLTs produced in vitro from non-treated and irradiated MKs were both able to adhere to fibrinogen-coated surfaces (e). Graphs are depicted as mean ± SD of n = 3 in (b,c) and n = 7 in (d).
