Figure 6
From: Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors
Megakaryocytes differentiated in stirred bioreactors produce PLTs after transfusion in a mouse model. MKs produced from iPSCs in stirred cell-OA or cell-MC cultivation, both non-treated and gamma-irradiated were transfused to NOD/SCID/IL-2Rγc−/− mice. After 1 h and 4 h, blood was drawn and analyzed for human PLTs. The gate shows human CD42a+CD61+ cells within the mouse blood cell population. The graph (a) and representative plots (b) show percentages of CD42a+CD61+ cells. Graph is depicted as mean ± SD of n = 4.
