Figure 2 | Scientific Reports

Figure 2

From: Naturally derived Heme-Oxygenase 1 inducers attenuate inflammatory responses in human dendritic cells and T cells: relevance for psoriasis treatment

Figure 2

Treatment of LPS-stimulated DC with carnosol or curcumin down-modulates expression of CD40, CD80, CD83 and CD86. DC from healthy donors (n = 6) were incubated with increasing doses of carnosol (2.5–10 µM) or curcumin (2.5–10 µM) for 6 hours prior to stimulation with LPS (100 ng/ml). Cells were stained with fluorochrome conjugated antibodies specific for CD40, CD80, CD83 and CD86 after 24 hours and analysed by flow cytometry. (A) Histograms depicting expression of maturation markers in carnosol and curcumin treated DC compared to vehicle control from one representative experiment. (B) Pooled data (n = 6) depicting expression of CD40, CD80, CD83 and CD86 in carnosol and curcumin treated DC. Results shown are mean (±SEM) of the measured Mean Fluorescence Intensities (MFI), expressed as percentages of the vehicle controls. Statistical significance was determined by one-way ANOVA, with Dunnett’s multiple comparisons post hoc test to compare treatment groups against the control group. (***p < 0.001, **p < 0.01, *p < 0.05).

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