Figure 5 | Scientific Reports

Figure 5

From: Naturally derived Heme-Oxygenase 1 inducers attenuate inflammatory responses in human dendritic cells and T cells: relevance for psoriasis treatment

Figure 5

Carnosol and curcumin reduce activation of MAP Kinases, and curcumin reduces nuclear translocation of the NFκB subunit p65, in LPS-stimulated DC. DC from healthy donors (n = 6) were incubated with carnosol (10 µM), curcumin (10 µM) or a vehicle control for 6 hours prior to stimulation with LPS for 15 minutes to 3 hours. (A) The activation of the MAPKs MEK, ERK and p38, as well as degradation of IκB was measured by western blot. Data shown is representative of 6 healthy donors. Full length blots are presented in Supplemental Figs 4 and 5 (B) The nuclear translocation of the NFκB subunit p65 in vehicle control, carnosol or curcumin treated DC after stimulation with LPS for 30 minutes was assessed by confocal microscopy. Data shown is from one healthy donor and is representative of 4 healthy donors. Cropped images are presented for clarity; full size images are presented in Supplemental Fig. 6.

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