Figure 3
From: The functional and inflammatory response of brain endothelial cells to Toll-Like Receptor agonists
Modelling of hCMVEC adhesion following Poly(I:C), LPS and Imiquimod treatment. TLR agonists Imiquimod (5 µg/mL) LPS (100 ng/ml), Poly(I:C) (5 µg/mL), or media-only control were administered to confluent hCMVECs 48 hours post seeding (represented by a red arrow) on an ECIS 96W20idf plate. Data shown has been normalized (100%) prior to the addition of ligands (raw data shown in Supplemental Fig. 3). Multi-frequency measurements were taken and this data was modelled using ECIS Software V.1.2.163.0 PC. (a) Shows the modelled intercellular adhesion (Rb) whilst (b) shows Rb 4, 24 and 48 hours post-treatment. (c) Shows the modelled basolateral adhesion (α). (d) Shows α at 4, 24, and 48 hours post-treatment for statistical comparisons. The modelled data is from a single experiment where each curve represents the mean ± SD of three individual wells. Representative of at least three independent experiments. Significance compared to control (unless otherwise stated). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
