Figure 7
From: The functional and inflammatory response of brain endothelial cells to Toll-Like Receptor agonists
Characterization of hCMVEC secretion 72 hours following administration of Poly(I:C) (5 µg/mL), Imiquimod (5 µg/mL), LPS (100 ng/ml), LPS (100 ng/ml) and Imiquimod (5 µg/mL), Poly(I:C) (5 µg/mL) and Imiquimod (5 µg/mL), and Poly(I:C) (5 µg/mL) and LPS (100 ng/ml) or untreated media-only control post-treatment. The concentration of soluble factors in the conditioned media was measured using multiplexed cytometric bead array. Cultured media was collected at 72 hours following treatment and assayed using a cytokine panel chosen based on previous research17. Data shows the mean ± SD (n = 3). Significance is compared to control unless otherwise indicated by the horizontal linking bars. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
