Figure 3
From: Exposure to podophyllotoxin inhibits oocyte meiosis by disturbing meiotic spindle formation
Podophyllotoxin exposure affected spindle formation and chromosome alignment in meiosis II of oocytes. (A) Fluorescence staining for the spindle and chromosomes after podophyllotoxin exposure. In the control group, the oocytes extruded the first polar body, the spindle showed well barrel shape morphology, and the chromosomes aligned well. However, after podophyllotoxin exposure the spindle morphology was disrupted, the chromosomes were mis-aligned. Similar with meiosis I, the fluorescence signals of microtubule were much weaker compared with the control group. Green, alpha-tubulin; red, chromosomes. Bar = 20 μm. (B) Rates of abnormal spindle morphology in the podophyllotoxin exposure oocyte group. *The statistical test was performed (p < 0.05). (C) Intensity of alpha-tubulin fluorescence signals in the podophyllotoxin exposure oocyte group. *The statistical test was performed (p < 0.05). (D) Rates of misaligned chromosomes in the podophyllotoxin exposure oocyte group. *The statistical test was performed (p < 0.05).
