Figure 1
From: A Mouse Model of Schnyder Corneal Dystrophy with the N100S Point Mutation
(A) Create an N100S mutation using CRISPR-Cas9 system with sgRNA sequence: GTATGTGTTGACCAAATTGCCGG. Four nucleotide substitutions are shown in lower case, N100S mutation is highlighted in red. PAM is highlighted as green. (B) To identify the genotypes of Ubiad1N100S mice, a 420 bp PCR product was amplified from purified tail DNA and subjected to HincII digestion. Homozygotes (Ubiad1N100S/N100S), heterozygotes (Ubiad1N100S/WT) and Ubiad1WT/WT have 1 (size 420 bp), 3 (size 420 bp, 287 bp and 133 bp), and 2 (size 287 bp and 133 bp) bands respectively after HincII digestion. WT, wild-type; Het, heterozygous; Homo, homozygous.
