Table 1 sgRNA sequence and cleavage efficiency.
From: A Mouse Model of Schnyder Corneal Dystrophy with the N100S Point Mutation
Guide RNA | Sequence | Efficiency (%) |
|---|---|---|
sgRNA1 | TGTACACGGGGCCGGCAATTTGG | 8.6 |
sgRNA2 | GTCCTGGCTGTACACGGGGCCGG | 12.3 |
sgRNA3 | GTATGTGTTGACCAAATTGCCGG | 12.9 |
Tet2 (control) | GAAAGTGCCAACAGATATCCAGG | 13.4 |
