Figure 4

qRT-PCR expression analyses of 6 transcription factor and 6 heat shock proteins in response to heat stress. qRT-PCR was performed to validate the results of the RNA sequencing analysis using cDNAs prepared from 3-year-old needles of Korean fir exposed for 21-day to control (22 °C) or heat-treatment (30 °C) conditions. Error bars denote standard errors of biological replicates. Expression values of each gene are normalised against the expression of Actin18.