Figure 4 | Scientific Reports

Figure 4

From: Altered protein turnover signaling and myogenesis during impaired recovery of inflammation-induced muscle atrophy in emphysematous mice

Figure 4

UPS signaling in skeletal muscle is only slightly increased in emphysematous mice during recovery following pulmonary inflammation. Mice were intratracheally instilled with elastase to induce emphysema or vc, followed by a single bolus of LPS or vc. 72 hours after LPS gastrocnemius muscle was collected for evaluation of mRNA abundance of (a) MuRF1, (b) Atrogin-1, (c) SMART, (d) MUSA1 and (h) FoXO1, normalized to GeNorm and expressed as fold change compared to control. Alternatively, protein levels of (f) phosphorylated FoXO1 (Thr24), (g) total FoXO1, (j) phosphorylated FoXO3A (Thr32) and (k) total FoXO3A were assessed. (l) Representative western blots of indicated proteins. Uncropped western blots are shown in Supplementary Fig. S4. Ratio of (e) phosphorylated FoXO1 over total FoXO1 and (i) phosphorylated FoXO3A over total FoXO3A. n = 7–8 per group. Significant differences were determined using an independent-samples T-test. Symbols above a bar refer to a comparison with the respective control (L vs. C and E + L vs. E). A symbol above a line refers to a comparison between the bars indicated by the line. *p < 0.05, **p < 0.01, ***p < 0.001. #represents a trend.

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