Figure 6 | Scientific Reports

Figure 6

From: Altered protein turnover signaling and myogenesis during impaired recovery of inflammation-induced muscle atrophy in emphysematous mice

Figure 6

Increased protein synthesis signaling in skeletal muscle of emphysematous but not control mice during recovery from pulmonary inflammation. Mice were intratracheally instilled with elastase to induce emphysema or vc, followed by a single bolus of LPS or vc. 72 hours after LPS gastrocnemius muscle was collected. Protein levels of (b) phosphorylated p70S6 (Thr389), (c) total p70S6, (f) phosphorylated S6 (ser235/236), (g) total S6, (i) phosphorylated 4EBP1(ser65) and (l) 4EBP1(Thr37/46) and (j) total 4EBP1 were assessed in lysates of gastrocnemius muscle tissue with western blot analysis. (d) Representative western blots of indicated proteins. Uncropped western blots are shown in Supplementary Fig. S6. Ratio of (a) phosphorylated p70S6 over total p70S6, (e) phosphorylated S6 over total S6 and (h) phosphorylated 4EBP1 (ser65) and (k) phosphorylated 4EBP1 (Thr37/46) over total 4EBP1. n = 7–8 per group. Significant differences were determined using an independent-samples T-test. Symbols above a bar refer to a comparison with the respective control (L vs. C and E + L vs. E). A symbol above a line refers to a comparison between the bars indicated by the line. *p < 0.05, **p < 0.01, ***p < 0.001, #represents a trend.

Back to article page