Figure 2 | Scientific Reports

Figure 2

From: Fibroblast growth factor 12 is expressed in spiral and vestibular ganglia and necessary for auditory and equilibrium function

Figure 2

Localisation of FGF12 mRNA and protein expression in the inner ear. (a–c) FGF12 mRNA localisation in the inner ear by in situ hybridisation. (b) The cochlea. (c) High-magnification image of (b), focused on the spiral ganglion. (d,e) In situ hybridisation images using the sense probe as a negative control. (f–j) Immunofluorescent staining for FGF12 (green) in the inner ear. (f) Low-magnification image of the inner ear. (g) Superior and inferior regions of the vestibular ganglion. (h) The spiral ganglion (SGN) and organ of Corti. White triangles indicate neural fibres. (i) Magnified and merged image of the organ of Corti. IHC, inner hair cell; OHC, outer hair cell. (j) The vestibular ganglion and sensory epithelium of the saccule. The triangle indicates the layer of supporting cells (SCs), which are characterised by cell alignment and rich cell density. (k) Image using FGF12-KO mouse tissue as a negative control. Phalloidin (red) visualises F-actin in hair cells. Hoechst® (blue) visualises the nucleus of each cell. H in (a–g) and (i–k), and 10 µm in (i).

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