Figure 4
From: Structural insights into ubiquitin phosphorylation by PINK1
Inhibition of parkin activation by structure-guided mutations of HsPINK1. Formation of the Parkin-Ub oxyester intermediate in the PINK1-knockout HeLa cells expressing HA-parkin (C431S) and wild-type or mutant HsPINK1-3xHA after CCCP treatment was examined by immunoblotting analysis using anti-parkin and anti-PINK1 antibodies.
