Figure 1

MT could inhibit acute and chronic pruritus. (A) Chemical structure of MT. (B–D) Dose-dependently inhibiting effects of MT (s.c.) on scratching bouts induced by 200 mM histamine, 8 mM chloroquine and 13 mM compound 48/80. (B) n = 5 in each group except n = 6 in 6 mg/kg MT group. (C) n = 7 in control and 30 mg/kg MT group and n = 5 in other group. (D) n = 5 in MT 3 mg/kg and 15 mg/kg group, n = 6 in other group. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, N.S., not significant. Tukey’s post-hoc analysis. (E) The time processing curves for the scratching behaviors of the animals treated with AEW (n = 18) and water (n = 5). The symptom of chronic itching was basically stable after the five days. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, unpaired student’s t-test. (F) 30 mg/kg MT (s.c.) (n = 7) significantly decreased the scratching behaviors in the AEW chronic pruritus model on the 7^th day compared to saline (n = 5) group. ***P ≤ 0.001, N.S., not significant, Tukey’s post-hoc analysis. (G) The time processing curves for the scratching behaviors of the animals treated with methods of AD model (n = 16) and olive oil (n = 8). The symptom of chronic itching was basically stable after nine days. **P ≤ 0.01, ***P ≤ 0.001, unpaired student’s t-test. (H) 30 mg/kg MT (s.c.) (n = 6) significantly decreased the scratching behaviors in the AD chronic pruritus model compared saline group (n = 5) on the 12^th day. ***P ≤ 0.001, N.S., not significant, Tukey’s post-hoc analysis.