Figure 2

MT did not influence the Ca²⁺ influx evoked by chloroquine and histamine and survival of DRG neurons. (A) The representative trace displayed intracellular Ca²⁺ influx induced by 1 mM chloroquine, and 100 µM MT showed no effect to DRG neurons. MT did not influence the baseline too. (B) MT did not change the intensity of chloroquine-induced calcium influx in DRG neurons, n = 25 in each group. N.S., not significant, paired student’s t-test. (C) The representative trace displayed intracellular calcium flux induced by 1 mM histamine, and 100 µM MT showed no effect on DRG neurons. (D) MT did not change the intensity of histamine-induced calcium influx in DRG neurons, n = 40 in each group. N.S., not significant, paired student’s t-test. (E) The MTT optical density of alive DRG neurons before (n = 5) and after being treated by pure DMSO (n = 3), 10 mM MT (n = 4), 1 mM MT (n = 4), 100 μM MT (n = 4) and 10 μM MT (n = 4). N.S., not significant. Tukey’s post-hoc analysis. (F) The dose-response curve of MT to inhibit the MTT optical density of alive HEK293 cells. n = 5 in each concentration. Group data presented by mean ± SEM.