Figure 5
From: Matrine inhibits itching by lowering the activity of calcium channel
MT inhibited HVA Ca2+ currents of N-type and L-type calcium channel. (A) The protocol of stimulus and traces of LVA ICa (−80 to −40 mV, 20 ms) and HVA ICa (−60 to 10 mV, 20 ms). Inward current traces were evoked by depolarization during bath application of MT and washed out. (B) Current-time curves of MT to inhibit the HVA ICa. (C) Histogram of HVA ICa current intensity during application and washout of MT, n = 10 in each group. **P ≤ 0.01, ***P ≤ 0.001, Tukey’s post-hoc analysis. (D) Stimulation protocols for inducing calcium channels and current traces under blockers of N-type (GVIA, 1 µM), L-type (nimodipine, 2 µM) and N-type + L-type. On the same neurons to run the protocol, the inhibitory effect of 100 µM MT (red line) was also detected and compared with the inhibitory effect of other calcium channels blockers. (E) The current-time curve relationships of three substances (MT, GVIA and nimodipine) to inhibit HVA ICa. (F) The inhibition percentage of N-type blockers (n = 12), L-type blockers (n = 11) and N- and L-type blockers together (n = 14) with and without MT perfusion to inhibit HVA Ica current. MT could inhibit the action of N-type and L-type calcium channels, but the inhibitory effect of MT on N-type was stronger than that of L-type. *P ≤ 0.05, ***P ≤ 0.001, N.S., not significant, paired student’s t-test. Group data presented by mean ± SEM.
