Figure 6

MT inhibited the frequency instead of amplitude of glutamatergic sEPSCs in lamina II neurons. (A) Patterns of electrode recording and stimulation sites were shown on spinal cord slice. The recording electrode was located on the substantia gelatinosa (SG, lamina II) neuron, and the stimulating electrode was located in the dorsal root of the spinal cord. (B) Spontaneous inward currents were recorded and the inhibitory effect of 100 μM MT on the substantia gelatinosa (SG, lamina II) neurons was shown. (C,D) Cumulative plots showed changes in the frequency and amplitude of glutamatergic sEPSCs after 100 μM MT perfusion in lamina II neurons. (E,F) The perfusion of MT reduced the firing frequency (n = 7) of the lamina II neurons, but did not change the amplitude (n = 5) of sEPSCs. ***P ≤ 0.001, N.S., not significant, paired student’s t-test. Group data presented by mean ± SEM.