Figure 7

MT inhibited the evoked synaptic responses in lamina II neurons, but the inward currents induced by exogenous glutamic acid were not affected. (A) The response traces of evoked excitatory postsynaptic currents (eEPSCs) to high-intensity, paired-pulse stimulation (500 µA, 0.1 ms, 400 ms interval) and application of 100 µM MT to lamina II neurons. The black and red lines indicated the response of the control and the 100 µM MT administration. (B) MT obviously decreased the eEPSC amplitude (n = 6). **P ≤ 0.01, paired student’s t-test. (C) MT significantly increased the paired-pulse ratio (PPR; 2^nd eEPSC amplitude/1^st eEPSC amplitude) (n = 6). **P ≤ 0.01, paired student’s t-test. (D) Whole-cell currents evoked by exogenous application of glutamate and 100 µM MT perfusion. (E) MT did not affect exogenous glutamate induced inward currents (n = 5). N.S., not significant, paired student’s t-test. Group data presented by mean ± SEM.