Figure 5
AU040320 localises to the TGN in developing spermatids. (A) Double immunohistochemistry for detection of endogenous AU040320 (custom antibody #78, red) and trans-Golgi marker TGN38 (green) shows co-localisation in spermatocytes and round spermatids. (B) Comparison of AU040320 localisation (#78, red) with the PNA pattern (green) over the course of acrosome development reveals association of the AU040320 protein with early acrosomal structures in Golgi phase spermatids but this seems to be reduced in Cap phase spermatids (white arrows); no clear signal for AU040320 can be detected at later stages (acrosome-maturation phases). (C) AU040320 protein (#78, red) exhibits a pattern with some overlap (arrows) with the structure of Sertoli cells (β-tubulin III, green), including a stronger juxta-nuclear signal compatible with TGN localisation (arrowhead), suggesting AU040320 is also present in these cells. For each condition, n = 3. Scale bars: 5 μm in all panels with the exception of in C (25 μm).
