Table 1 Antibodies and other detection reagents used in immunostaining experiments.
Antibody/reagenta | Conjugateb | Speciesc | Dilution | Company | Cat. No. |
|---|---|---|---|---|---|
anti-KIAA0319L (#78) | Guinea pig | 1:1000 | (custom made) | KL-FCt-G1 | |
anti-KIAA0319L (Rb-AU) | Rabbit | 1:1000 | ProteinTech | 21016-1-AP | |
anti-acetylated Tubulin [6-11B-1] | Mouse; M | 1:1000 | Sigma | T7451 | |
anti-beta-Tubulin [TUB2.1] | CY3 | Mouse; M | 1:200 | Sigma | C4585 |
anti-beta-III Tubulin [TUJ1] | Mouse; M | 1:1000 | Abcam | ab14545 | |
anti-phospho-Histone H2A.X (Ser139) [JBW301] | Mouse; M | 1:500 | Millipore | 05–636 | |
anti-SCP3 | Rabbit | 1:500 | Abcam | ab15093 | |
anti-rat TGN38 | Sheep | 1:200 | BioRad | AHP499 | |
anti-Sheep IgG (H + L) | Biotin | Rabbit | 1:100 | Vector Laboratories | BA-6000 |
anti-Guinea pig IgG (H + L) | AF-488 | Goat | 1:1000 | Molecular Probes | A11073 |
anti-Guinea pig IgG (H + L) | AF-568 | Goat | 1:1000 | Molecular Probes | A11075 |
anti-Rabbit IgG (H + L) | AF-488 | Goat | 1:1000 | Molecular Probes | A11008 |
anti-Rabbit IgG (H + L) | AF-568 | Goat | 1:1000 | Molecular Probes | A11011 |
anti-Mouse IgG (H + L) | AF-488 | Goat | 1:1000 | Molecular Probes | A11001 |
anti-Mouse IgG (H + L) | AF-568 | Goat | 1:1000 | Molecular Probes | A11004 |
Streptavidin | AF-488 | 1:500 | Molecular Probes | S11223 | |
Lectin PNA | AF-488 | 1:1000 | Molecular Probes | L21409 | |
Lectin PNA | AF-568 | 1:1000 | Molecular Probes | L32458 | |
MitoTracker | Deep Red FM | 1:1000 | Molecular Probes | M22426 |
