Figure 6
ESR1 binding is altered in CD treated testes. The ChIP was performed using antibody against ESR1 in E15.5 testis. The immunoprecipitated DNA was quantitated with fluorescent method and equal amount of ChIP and Input DNAs was taken for each PCR using the primers indicated in SI. The ESR1 predicted binding sites were obtained by FIMO analysis and primers were designed after repeat masking of each target sequence. The copy number for each gene was normalized for quantity to control region without ESR1 binding sites (Gapdh gene) and compared to Input. The data was expressed as ChIP-to-input enrichment.
