Figure 2
From: Dynamic behavior of DNA topoisomerase IIβ in response to DNA double-strand breaks
Live imaging of Topo IIβ recruitment to damaged sites. (A) Recruitment of EGFP-Topo IIβ to damaged sites. EGFP-Topo IIβ was transiently expressed in HeLa cells. DNA damage was induced with shots of a pulsed UVA laser, and recruitment of EGFP-Topo IIβ to the damaged sites was monitored by time-lapse microscopy. Representative images at the indicated time points are shown. (B) Time-course of EGFP-Topo IIβ recruitment to damaged sites. DNA damage was induced in the nucleus with a single shot of a pulsed UVA laser. After time-lapse imaging, EGFP signals in the damaged site were quantified, and average values with SD were calculated from 10 cells. (C) Recruitment of EGFP-Topo IIβ to DSB sites. Either EGFP-XRCC1 or EGFP-Topo IIβ was transiently expressed in HeLa cells. The cells were line-scanned with a 405 nm laser in the presence or absence of Hoechst 33342. Representative images at the indicated time points are shown.
