Figure 3
From: Dynamic behavior of DNA topoisomerase IIβ in response to DNA double-strand breaks
ATM- and DNA-PKcs-independent recruitment of Topo IIβ to DSB sites. (A) Recruitment of Topo IIβ to DSB sites in ATM-deficient cells. HeLa and ATM-deficient AT5 cells were microirradiated with a pulsed UVA laser. After 10 min incubation at 37 °C, the cells were fixed and immunostained for Topo IIβ and phosphorylated serine 1981 (pS1981) of ATM. (B) Recruitment of Topo IIβ to DSB sites in DNA-PKcs-deficient cells. The DNA-PKcs-proficient M059K and -deficient M059J cells were subjected to laser microirradiation followed by immunostaining for Topo IIβ and phosphorylated serine 2056 (pS2056) of DNA-PKcs.
