Figure 4
From: Quantitative fibre analysis of single-molecule localization microscopy data
IRIS imaging of the F-actin meshwork in T cell synapses. Representative IRIS images of a mature T cell synapse (Panel a) and a Cytochalasin D treated synapse (Panel b) with inset fibre analysis examples of 3 × 3 μm regions. Quantitation of untreated cells in both central and peripheral regions are shown for both control (n = 5 cells, n = 15 regions, (a,i–iv)) and actin disrupting (n = 3 cells, n = 15 regions, (b,i–iv)) conditions. Scale bar 5 μm. ns = not significant, *p < 0.05, **p < 0.005, ***p < 0.0005.
