Figure 4

Biotransformation of EsLeuDH-DM with aliphatic carbonyl compounds as substrates (2.50 µmol) in NH4Cl-buffer (50 mM, pH 9.5) utilizing EsLeuDH-DM (10 U), algae supernatant (3 mM), NAD+ (1 µmol) and cb-FDH (3.30 U) in a total volume of 2.3 mL at 30 °C.