Figure 5

Combination of microalgae-catalyzed formate production with enzymatic amine synthesis in a one-pot process. Biotransformations were carried out with n-butanal (1a, 0.18 mg, 2.50 µmol), n-hexanal (1d, 0.25 mg, 2.50 µmol), 2-butanone (1g, 0.18 mg, 2.50 µmol), cyclohexanone (1i, 0.25 mg, 2.50 µmol) in NH₄Cl-buffer (50 mM, pH 9.5), EsLeuDH-DM (10 U), C. reinhardtii cells cells (1 mL cell suspension in HSM medium; 10⁸ cells), NAD⁺ (1 µmol) and cb-FDH (3.30 U) in a total volume of 2.3 mL at 30 °C.