Figure 3
From: The Drosophila microbiome has a limited influence on sleep, activity, and courtship behaviors

Removal of Drosophila microbiota mildly increases locomotion in the F2 generation. (A) The average locomotion speed of F1 germ-free (GF) singly housed flies was elevated relative to conventional (CV) control animals (+0.09 mm/s [95CI −0.03, +0.21], g = 0.237, P = 1.002e-01, N = 75, 74). (B) F1 group housed GF flies displayed an increase in locomotion relative to CV controls (+0.07 mm/s [95CI −0.05, +0.18], g = 0.17, P = 3.357e-01, N = 96, 80). (C) Individually housed F2 GF flies walked 24.5% faster than controls (+0.31 mm/s [95CI + 0.15, +0.47], g = 0.608, P = 1.08 × 10−3, N = 57, 56). (D) Group housed F2 GF flies walked 36.5% faster than controls (+0.30 mm/s [95CI + 0.18, +0.41], g = 0.683, P = 8.5 × 10−5, N = 89, 66).