Figure 3

Loss of Ano2 does not impact respiration rate. (A) Example respiration traces from a Ano2^+/+, Ano2^−/− and C57BL/6 J animals recorded with a thermocouple placed near the animal’s nose (see Supplemental Fig. 2 for technique validation). (B) Histograms of the instantaneous respiration frequency in a 5-minute window from three representative animals from each group. Mean instantaneous frequency is displayed next to each plot. (C) Mean instantaneous frequency for all animals in each group. Red bars denote mean and standard error across all animals (p = 0.71, Kruskal-Wallis test).