Figure 2 | Scientific Reports

Figure 2

From: Vesicular stomatitis virus G protein transmembrane region is crucial for the hemi-fusion to full fusion transition

Figure 2

Replacement of the VSV G TM region with TM regions from other membrane fusion proteins resulted in similar protein expression levels and plasma membrane localization. (a) Sequences of the TM regions of VSV G, Syntaxin 1 A, VAMP2 and influenza HA. (b and c) VSV G TM replacement did not lead to dramatic changes in the expression levels of VSV G in HeLa cells. (d) WT and chimeric VSV G localized onto the plasma membrane. HeLa cells were transfected with plasmids encoding WT or chimeric VSV G proteins fused with EGFP. (e and f) Biotinylation assays to quantify membrane-localized WT or chimeric VSV G proteins. Total VSV G levels in cells (I: input) and membrane-localized G protein levels (P: pull down by streptavidin resin) were determined by western blot. The ratio of biotinylated VSV G to total G protein was calculated. Compared to WT VSV G, chimeric mutants showed similar levels of membrane localization. Mean ± SEM.

Back to article page