Figure 3
From: 3D surface morphology imaging of opaque microstructures via light-field microscopy

3D micro-scaffold difference of height measurement for low contrast bright filed image. (a) CMOS sensor image of two 3D cubic, the area is 75 × 75 μm2, and height are 40 μm (blocked by blue dot square) and 50 μm (blocked by red dot square) respectively. (b) Digital refocused image of part (a) at different depth from 0μm to 33.7 μm. (c) Image quality versus alpha value curve analysed from two ROI (blue and red dot block of part (a)), from the results of part (c), two best digital refocused images are marked in part (b) with blue and red dot blocks (correspond to the color of curves).