Figure 5
CRF patient erythrocytes show both enhanced eryptosis and VWF binding. (A) Percentage of AV-positive erythrocytes in healthy control individuals (left bar; n = 5) and CRF patients (right bar; n = 5) measured by FACS from freshly isolated erythrocytes. The right table shows CRF patient blood parameters regarding anemia. The arrows indicate elevated or decreased values compared with normal values. (B) Calculation of erythrocyte lifespan in healthy control individuals (left bar; n = 5) and in CRF patients (right bar; n = 5) based on either AV-positivity (left graph) or hemoglobin content (right graph). For the first calculation, the formula (100%*eryptosis rate(patient)/eryptosis rate(control) = %lifespan of patient erythrocytes) was used; for the second calculation, the formula (100% * hemoglobin(patient)/hemoglobin(control) = %lifespan of patient erythrocytes) was used. (C) Quantification of spontaneous adherence of erythrocytes from healthy control donors (white bars) and CRF patients (black bars) in a VWF-coated flow channel visualized by RICM (n = 5). (D) Quantification and (E) representative RICM images of spontaneous erythrocyte adherence of CRF patient samples that were untreated (control) or supplemented with Annexin V (AV), an anti-Annexin V antibody (α-AV) or heparin (Hep), as indicated, after 10 minutes of flow at 1.5 dyne/cm² (n = 4). Scale bars correspond to 20 µm. *p < 0.05; **p < 0.01 (two-sided Student’s t test).
