Figure 1

Analysis of infarcted area after MKEY treatment. Area at risk (AAR) as % total area (A) and infarcted area as % of AAR (B) were determined by Evans-blue perfusion and tetrazolium staining 1d after injury in control and MKEY-treated mice. (C) Representative heart sections from both groups are shown. *p < 0.05 vs. control (n = 6); (D) Quantification of the number of apoptotic cells as determined by TUNEL staining in MKEY- and control-treated mice. *p < 0.05 vs. control (n = 8); (E,F) Representative images of TUNEL staining of infarct tissue. Scale bar 50 µm. (E) TUNEL-positive cells are green, inset: control staining. (F) TUNEL-positive cells are red and cardiomyocytes are visualised using α-actinin (green). Nuclei were stained using DAPI (blue).