Table 4 Oligonucleotide Primers used in this Study.
Purpose | Primer | Sequencea |
|---|---|---|
Construction of UCC2003 nagA1 deletion | nagA1SOE A | catctggtgctgctcgctttcg |
nagA1SOE B | agtgagcagcacgtcggcggccgccacatcgatgccatccg | |
nagA1SOE C | cggatggcatcgatgtggcggccgccgacgtgctgctcac | |
nagA1SOE D | ctcaaggctgcgatcgacatg | |
nagA1SOE E | cgctcactgcagcacccgcacgccacgatcatc | |
nagA1SOE F | atctccctgcagctcaaggctgcgatcgacatg | |
Construction of UCC2003 nagA2 deletion | nagA2SOE A | gcatccgcacgccacgattatc |
nagA2SOE B | caccaaacctgttcgaccgtccaatagccaggagtcaggattcg | |
nagA2SOE C | cgaatcctgactcctggctattggacggtcgaacaggtttggtg | |
nagA2SOE D | gatctacggcatcaatgagc | |
nagA2SOE E | aactgcctgcagcgctacgcatacacccacaag | |
nagA2SOE F | atctccctgcagatcattcgttcctgtgcgctttg | |
pBS423 multiple cloning site primers | pBSF | cgttacgttattagttat |
pBSR | gtaatacgttcgtgtcgcg | |
Amplification of spectinomycin resistance cassette | specFw | gtcgtcgtatctgaacc |
specRv | gataactacgaactgctaac |
