Figure 1

Robo receptors are required for endocrine cell type sorting and mature architecture of the islets of Langerhans. (A) Immunofluorescence staining for β cells (Insulin, red) and α cells (glucagon, green) of control (Robo1^+/Δ,2^+/flx and Neurog3-Cre;Robo1^+/+,2^+/+) and Neurog3-Cre;Robo1^Δ/Δ,2^flx/flx islets from 2 month old mice. (B) Average Circularity Index of control (Robo1^+/Δ,2^+/flx and Neurog3-Cre;Robo1^+/+,2^+/+) and Neurog3-Cre;Robo1^Δ/Δ,2^flx/flx islets. (C) Percentage of total α cells found in periphery of the islet in control (Robo1^+/Δ,2^+/flx and Neurog3-Cre;Robo1^+/+,2^+/+) vs. Neurog3-Cre;Robo1^Δ/Δ,2^flx/flx mice. (D) Average size of control (Robo1^+/Δ,2^+/flx and Neurog3-Cre;Robo1^+/+,2^+/+) and Neurog3-Cre;Robo1^Δ/Δ,2^flx/flx. (E) Percent of α cells out of total cells in control (Robo1^+/Δ,2^+/flx and Neurog3-Cre;Robo1^+/+,2^+/+) and Neurog3-Cre;Robo1^Δ/Δ,2^flx/flx.