Figure 4

The disrupted islet architecture and increase in α cell ratio in Robo mβKO islets is not due to defects in differentiation or maturation of β cells. (A) Immunofluorescent staining for Insulin (red) and Ucn3 (green) of control Ucn3-Cre;Robo1+/+,2+/+ and Ucn3-Cre;Robo1Δ/Δ,2flx/flx islets from 1.5–3 month old mice. (B) Immunofluorescent staining for Insulin (red) and MafA (green) of control Ucn3-Cre;Robo1+/+,2+/+ and Ucn3-Cre;Robo1Δ/Δ,2flx/flx islets from 1.5–3 months old mice. Disorganized islets do not show loss of β cell maturation.