Figure 3

Label-free quantitative proteomic analysis by mass spectometry of urine-derived cells from Fabry patients. (A) Hierarchical clustering of proteins (label-free quantification (LFQ) values) within 4 Fabry patient and 4 control cell samples based on Euclidean distance. Heat map displays normalized log2 LFQ intensities of all n = 3646 proteins quantified across the samples (red = high, blue = low intensity). (B) Volcano plot showing logarithmized fold change of LFQ values measured from Fabry patient vs. control samples. Ratios plotted against the negative logarithmic P value of the Student’s t-test. Each dot represents a protein. α-Gal (gene GLA) was the most significantly and strongly decreased protein in Fabry patient cells. Proteins with a >2 fold difference (log2 ratio > 1 or < −1) are marked in blue. Proteins with a >2 fold difference and p < 0.05 are marked in blue including selected gene names. The comprehensive list is provided in Table 2. (C) The same Volcano plot as in B with lysosomal lumen proteins (GOCC) depicted in black.