Figure 2 | Scientific Reports

Figure 2

From: Prevascularization of dermal substitutes with adipose tissue-derived microvascular fragments enhances early skin grafting

Figure 2

Histology and immunohistochemistry. (a and b) HE-stained sections of non-seeded (a) and prevascularized (b) implants. Broken line = implant border, arrowheads = mesh fibers. (ce) Polarized light microscopy of Sirius red-stained sections of normal skin (c), non-seeded (d) and prevascularized (e) Integra. (f) Quantification of total collagen ratio (implant/skin). Mean ± SEM, n = 8, **P < 0.001 vs. non-seeded control. (gi and km) Immunohistochemical staining of microvessels (gi, white arrowhead = CD31+/GFP+ microvessel) and lymphatic vessels (km, white arrowhead = LYVE-1+/GFP+ lymphatic vessel, empty arrowhead = LYVE-1+/GFP lymphatic vessel) within prevascularized Integra 21 days after implantation. (j and n) Quantification of microvessel density (mm−2) and lymphatic vessel density (mm−2). Mean ± SEM, n = 8, **P < 0.001 vs. non-seeded control. Scale bars: a and b = 800 µm, ce = 20 µm, gi = 20 µm, km = 30 µm.

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